MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1225 . THIOALKALIBACTER HALOPHILUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1225 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 95 g 95 896.328
NaHCO3 15 g 15 178.557
NaCl 16 g 16 273.785
K2HPO4 1 g 1 5.741
Distilled water 1000 ml - -
1 Sterilize at 120°C 20 min in a closed vessel (i.e. a serum tube or bottle) with an air to liquid ratio of 10:1. pH after sterilization will be about 10.
2 After sterilization add:
Trace element solution 1 ml/l - -
MgCl2 x 6 H2O
(200.0 g/l) 		1 ml/l 0.2 0.984
3 (a white colloid will form which will rapidly dissolve after mixing)
Sodium thiosulfate pentahydrate
(1.0 M - 248.0 g/l) 		20 ml/l 4.964 20
NH4Cl
(1 M - 53.5 g/l) 		4 ml/l 0.214 4

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 925).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5 mg 0.005 0.017
FeSO4 x 7 H2O 2 mg 0.002 0.007
ZnSO4 x 7 H2O 100 mg 0.1 0.348
MnCl2 x 4 H2O 30 mg 0.03 0.152
CoCl2 x 6 H2O 200 mg 0.2 1.54
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 30 mg 0.03 0.124
CuCl2 x 2 H2O 10 mg 0.01 0.059
H3BO3 300 mg 0.3 4.851
Distilled water 1000 ml - -
1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min.

Metadata

Last modified: 08.06.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 95 896.328
NaCl 16 273.785
NaHCO3 15 178.557
Sodium thiosulfate pentahydrate 4.964 20
K2HPO4 1 5.741
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain