Name: THIOHALOSPIRA ALKALIPHILA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1229 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	120.0	g	120	2053.39
K₂HPO₄	2.0	g	2	11.483
(NH₄)₂SO₄	0.5	g	0.5	3.784
Distilled water	1000.0	ml	-	-
1 Flush the gas phase with nitrogen and add 5% oxygen. Sterilize at 120°C 20 min in a closed vessel (i.e. a serum tube or bottle) with a liquid to air ratio of about 1:10.
2 After sterilization add from sterile stock solutions:
Trace element solution	1.0	ml	-	-
MgCl₂ x 6 H₂O (200.0 g/l)	2.0	ml	-	-
3 (a white colloid will form which will rapidly dissolve after mixing)
Sodium thiosulfate pentahydrate (4 M - 992 g/l)	5.0	ml	4.964	20
Vitamin B₁₂ (filter sterilized 100mg/l)	1.0	ml	-	-
NaHCO₃ (84.0 g/l)	100.0	ml	-	-
4 Final pH is 8.0 - 8.5.
5 Incubate statically until growth occurs, then shake at 100 rpm.

Trace element solution #

1000

Solution occurs in 5 other media (first defined in Medium 925).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA	5	mg	0.005	0.017
FeSO₄ x 7 H₂O	2	mg	0.002	0.007
ZnSO₄ x 7 H₂O	100	mg	0.1	0.348
MnCl₂ x 4 H₂O	30	mg	0.03	0.152
CoCl₂ x 6 H₂O	200	mg	0.2	1.54
NiCl₂ x 6 H₂O	20	mg	0.02	0.154
Na₂MoO₄ x 2 H₂O	30	mg	0.03	0.124
CuCl₂ x 2 H₂O	10	mg	0.01	0.059
H₃BO₃	300	mg	0.3	4.851
Distilled water	1000	ml	-	-
1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min.

All strains for this medium:	1
Strains with modifications:	1

Compound	Conc. [g/L]	Conc. [mM]
NaCl	120	2053.39
Sodium thiosulfate pentahydrate	4.964	20
K₂HPO₄	2	11.483
(NH₄)₂SO₄	0.5	3.784
H₃BO₃	3.0e-4	0.005
CoCl₂ x 6 H₂O	2.0e-4	0.002
ZnSO₄ x 7 H₂O	1.0e-4	3.5e-4
MnCl₂ x 4 H₂O	3.0e-5	1.5e-4
Na₂MoO₄ x 2 H₂O	3.0e-5	1.2e-4
NiCl₂ x 6 H₂O	2.0e-5	1.5e-4
CuCl₂ x 2 H₂O	1.0e-5	5.9e-5
EDTA	5.0e-6	1.7e-5
FeSO₄ x 7 H₂O	2.0e-6	7.2e-6
MgCl₂ x 6 H₂O	-	-
Distilled water	-	-
Vitamin B₁₂	-	-
NaHCO₃	-	-
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