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ACIDITHIOBACILLUS FERRIVORANS MEDIUM
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Equipment needed:
Autoclave
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Final pH:
n.d.
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2SO4 | 1.40 | g | ||
|
|
(NH4)2SO4 | 3.00 | g | ||
|
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KCl | 0.10 | g | ||
|
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MgSO4 x 7 H2O | 0.50 | g | ||
|
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K2HPO4 | 0.05 | g | ||
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Ca(NO3)2 x 4 H2O
(0.1% w/v) |
20.00 | ml | ||
|
|
(NH4)2Ni(SO4)2 x 6 H2O
(0.1% w/v) |
20.00 | ml | ||
|
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Modified Wolin's mineral solution | 10.00 | ml | ||
|
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K2S4O6 | 0.80 | g | ||
|
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FeSO4 x 7 H2O | 0.03 | g | ||
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Distilled water | 950.00 | ml | ||
| 1 Dissolve ingredients (except tetrathionate and ferrous sulfate), adjust pH to 2.5 with H2SO4 and autoclave. After cooling, add tetrathionate from a 100 mM stock solution and FeSO4 from a 10 mM stock solution (prepared in water adjusted to pH 2.0 with H2SO4). Both solutions should be freshly prepared and sterilized by filtration. | |||||
Modified Wolin's mineral solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
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MnSO4 x H2O | 0.50 | g | ||
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NaCl | 1.00 | g | ||
|
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FeSO4 x 7 H2O | 0.10 | g | ||
|
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CoSO4 x 7 H2O | 0.18 | g | ||
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CaCl2 x 2 H2O | 0.10 | g | ||
|
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ZnSO4 x 7 H2O | 0.18 | g | ||
|
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CuSO4 x 5 H2O | 0.01 | g | ||
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AlK(SO4)2 x 12 H2O | 0.02 | g | ||
|
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H3BO3 | 0.01 | g | ||
|
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Na2MoO4 x 2 H2O | 0.01 | g | ||
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NiCl2 x 6 H2O | 0.03 | g | ||
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Na2SeO3 x 5 H2O | 0.30 | mg | ||
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Na2WO4 x 2 H2O | 0.40 | mg | ||
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Distilled water | 1000.00 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||