This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaHCO3 | 30.0 | g | 30 | 357.113 | |
NaCl | 6.0 | g | 6 | 102.669 | |
K2HPO4 | 0.5 | g | 0.5 | 2.871 | |
Na2CO3 | 5.3 | g | 5.3 | 50.006 | |
Trace element solution SL-12 B | 1.0 | ml | - | - | |
Distilled water | 1000.0 | ml | - | - | |
MgSO4 | 1.0 | mM | 0.12 | 1 | |
Yeast extract | 50.0 | mg | 0.05 | - | |
Sodium acetate | 20.0 | mM | 1.641 | 20 | |
KNO3 | 20.0 | mM | 2.022 | 20 | |
Na2S | 0.1 | mM | 0.008 | 0.1 | |
1 Flush the medium with N2 and dispense 20 ml into 100ml serum bottles under an N2 atmosphere, seal with butyl rubber stoppers and autoclave. To the cooled. sterile medium add, from anaerobic, sterile stock solutions, MgSO4 (1 mM), yeast extract (50 mg/l), sodium acetate (20 mM), KNO3 (20 mM) and Na2S (0.1 mM). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 3.0 | g | 3 | 8.923 | |
FeSO4 x 7 H2O | 1.1 | g | 1.1 | 3.957 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
MnCl2 x 2 H2O | 50.0 | mg | 0.05 | - | |
ZnCl2 | 42.0 | mg | 0.042 | 0.308 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 18.0 | mg | 0.018 | 0.074 | |
H3BO3 | 300.0 | mg | 0.3 | 4.851 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
Distilled water | 1000.0 | ml | - | - | |
1 Adjust pH to 6.0. |
Last modified: | 24.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter, Ice bath |
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All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 30 | 357.113 |
NaCl | 6 | 102.669 |
Na2CO3 | 5.3 | 50.006 |
KNO3 | 2.022 | 20 |
Sodium acetate | 1.641 | 20 |
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