123 .
MARINE AGAR
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Selected strain: Psychrobacillus insolitus DSM 5
This strain uses the standard medium without modifications.
Growth conditions: 20 °C
Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Synthetic sea water | 1000 | ml | ||
|
|
Bacto peptone | 5 | g | ||
|
|
Yeast extract
(Bacto) |
1 | g | ||
|
|
Agar | 15 | g | ||
| 1 To 1000 ml "Synthetic sea water" (see medium 79) add 5 g Bacto Peptone, 1 g Bacto Yeast extract, and 15 g agar. Alternatively medium 514 may be used. | |||||
Synthetic sea water #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 24.00 | g | ||
|
|
MgCl2 x 6 H2O | 11.00 | g | ||
|
|
Na2SO4 | 4.00 | g | ||
|
|
CaCl2 x 6 H2O | 2.00 | g | ||
|
|
KCl | 0.70 | g | ||
|
|
KBr | 0.10 | g | ||
|
|
H3BO3 | 0.03 | g | ||
|
|
NaSiO3 x 9 H2O | 5.00 | mg | ||
|
|
SrCl2 x 6 H2O | 0.04 | g | ||
|
|
NaF | 3.00 | mg | ||
|
|
NH4NO3 | 2.00 | mg | ||
|
|
Fe(III)PO4 x 4 H2O | 1.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.8. | |||||