DSMZ 123 . MARINE AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
* Modified for Vibrio harveyi DSM 2332

Rehydrate and grow freeze-dried cells from the ampoule in liquid medium 123. Subsequent subculturing may be carried out in liquid medium and on agar plates

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Synthetic sea water 1000 ml - -
Bacto peptone 5 g 5 -
Yeast extract
(Bacto)
1 g 1 -
Agar 15 g 15 44.599
1 To 1000 ml "Synthetic sea water" (see medium 79) add 5 g Bacto Peptone, 1 g Bacto Yeast extract, and 15 g agar. Alternatively medium 514 may be used.
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 24.00 g 24 410.678
MgCl2 x 6 H2O 11.00 g 11 54.106
Na2SO4 4.00 g 4 28.161
CaCl2 x 6 H2O 2.00 g 2 9.129
KCl 0.70 g 0.7 9.39
KBr 0.10 g 0.1 0.84
H3BO3 0.03 g 0.03 0.485
NaSiO3 x 9 H2O 5.00 mg 0.005 -
SrCl2 x 6 H2O 0.04 g 0.04 0.15
NaF 3.00 mg 0.003 0.071
NH4NO3 2.00 mg 0.002 -
Fe(III)PO4 x 4 H2O 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
1 Adjust pH to 7.8.

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 34
Strains with modifications: 3
Vibrio harveyi DSM 2332

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24 410.678
Agar 15 44.599
MgCl2 x 6 H2O 11 54.106
Bacto peptone 5 -
Na2SO4 4 28.161
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Cultivation metadata from 34 strains BacDive

halophily moderately halophilic 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 3 34 strains
nutrition chemoorganotroph 1 strain
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