DSMZ 124 . DESULFOFARCIMEN MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.17 g 1.17 20.021
MgCl2 x 6 H2O 0.40 g 0.4 1.968
KCl 0.30 g 0.3 4.024
CaCl2 x 2 H2O 0.15 g 0.15 1.02
NH4Cl 0.27 g 0.27 5.048
KH2PO4 0.20 g 0.2 1.47
Na2SO4 2.84 g 2.84 19.994
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Na-acetate 1.40 g 1.4 17.066
Na-butyrate 1.40 g 1.4 12.717
Yeast extract 1.00 g 1 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
NaHCO3 4.50 g 4.5 53.567
Wolin's vitamin solution 10.00 ml - -
Na2S x 9 H2O 0.40 g 0.4 1.665
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, vitamins, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 7.0, then dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.0 - 7.2, if necessary.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 6
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.5 53.567
Na2SO4 2.84 19.994
Na-acetate 1.4 17.066
Na-butyrate 1.4 12.717
NaCl 1.17 20.021
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Cultivation metadata from 6 strains BacDive

pH 6 - 8 1 strain
phyla 1 6 strains
oxygen anaerobe 5 strains
temperature mesophilic 4 strains
temperature thermophilic 2 strains