Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
NH4Cl | 1.0 | g | ||
|
KH2PO4 | 0.3 | g | ||
|
K2HPO4 | 0.3 | g | ||
|
KCl | 0.1 | g | ||
|
NaCl | 1.0 | g | ||
|
NaNO3 | 1.5 | g | ||
|
CaCl2 x 2 H2O | 0.1 | g | ||
|
MgCl2 x 6 H2O | 0.5 | g | ||
|
Modified Wolin's mineral solution | 10.0 | ml | ||
|
Yeast extract | 1.0 | g | ||
|
Na2CO3 | 1.0 | g | ||
|
D-Glucose | 3.5 | g | ||
|
Distilled water | 1000.0 | ml | ||
1 Dissolve ingredients (except carbonate and glucose), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add glucose from a sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The pH of the complete medium should be 7.0. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
MnSO4 x H2O | 0.50 | g | ||
|
NaCl | 1.00 | g | ||
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
CoSO4 x 7 H2O | 0.18 | g | ||
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
ZnSO4 x 7 H2O | 0.18 | g | ||
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
AlK(SO4)2 x 12 H2O | 0.02 | g | ||
|
H3BO3 | 0.01 | g | ||
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
Na2SeO3 x 5 H2O | 0.30 | mg | ||
|
Na2WO4 x 2 H2O | 0.40 | mg | ||
|
Distilled water | 1000.00 | ml | ||
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |