1253
1253 .
LACTOBACILLUS KEFIRANOFACIENS
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Lactobacillus kefiranofaciens subsp. kefiranofaciens DSM 5016
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 1253 #
ml
Equipment needed: Autoclave
Wheaton tubes
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Man Rogosa Sharp broth
(MRS, Difco) |
55 | g | 54.402 | - | |
| Maltose | 10 | g | 9.891 | 28.896 | |
| Distilled water | 900 | ml | - | - | |
|
White wine
(filter sterilized) |
100 | ml | - | - | |
| Hemin solution | 10 | ml | - | - | |
|
Cysteine hydrochloride solution (10%)
(10%) |
1 | ml | 0.1 | 0.634 | |
| 1 Add all products except white wine, hemin and cysteine solutions. Adjust pH to 6.5 and autoclave for 15 minutes. Cool to 50°C and add aseptically white wine, hemin and cysteine solutions. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Hemin | 50 | mg | 0.5 | 0.767 | |
|
NaOH
(1N) |
1 | ml | - | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Dissolve 50 mg hemin in 1 ml 1N NaOH; make to 100 ml with distilled water. Autoclave at 121°C for 15 min. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Cysteine hydrochloride | 10 | g | 100 | 634.437 | |
| Distilled water | 100 | ml | - | - | |
| 1 Filter sterilize. | |||||
Metadata
| Last modified: | 27.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave, Filter, Hungate tubes, Wheaton tubes |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Lactobacillus kefiranofaciens subsp. kefiranofaciens DSM 5016
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Man Rogosa Sharp broth | 54.402 | - |
| Maltose | 9.891 | 28.896 |
| Cysteine hydrochloride | 0.099 | 0.628 |
| Hemin | 0.005 | 0.008 |
| White wine | - | - |
| Show more | ||
