125 .
METHYLOBACTERIUM MEDIUM
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Equipment needed:
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KNO3 | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
CaCl2 x 2 H2O | 0.02 | g | ||
|
|
Na2HPO4 | 0.23 | g | ||
|
|
NaH2PO4 | 0.07 | g | ||
|
|
FeSO4 x 7 H2O | 1.00 | mg | ||
|
|
CuSO4 x 5 H2O | 5.00 | µg | ||
|
|
H3BO3 | 10.00 | µg | ||
|
|
MnSO4 x 5 H2O | 10.00 | µg | ||
|
|
ZnSO4 x 7 H2O | 70.00 | µg | ||
|
|
MoO3 | 10.00 | µg | ||
|
|
Agar | 12.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Add 5 ml methanol or incubate under methane-air (4:1). Adjust pH to 6.8. | |||||