1262
1262 .
CM + YE MEDIUM + 10% NACL
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1262 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casamino acids
(vitamin free, Difco) |
7.5 | g | 7.5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| MgSO4 x 7 H2O | 20.0 | g | 20 | 81.144 | |
| Na3-citrate | 3.0 | g | 3 | 11.625 | |
| KCl | 2.0 | g | 2 | 26.828 | |
| NaCl | 200.0 | g | 200 | 3422.31 | |
| Fe SO4 x 7 H2O | 1.0 | ml | - | - | |
| Agar | 15.0 | g | 15 | 44.599 | |
|
NaCl
(10%) |
100.0 | g | 100 | 1711.16 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 pH 7.4 (NaOH) | |||||
| 2 Prepare 4.98% FeSO4 x 7 H2O (= 10 g Fe in 1l 0.001 M HCl); add 1 ml per liter medium. | |||||
Metadata
| Last modified: | 19.09.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 7.4 |
| Edit in Medium builder | |