1263
1263 .
SPIROCHAETA DISSIPATITROPHA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1263 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 30 | 513.347 | |
| KCl | 0.20 | g | 0.2 | 2.683 | |
| K2HPO4 | 0.20 | g | 0.2 | 1.148 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2CO3 | 2.76 | g | 2.76 | 26.041 | |
| NaHCO3 | 24.00 | g | 24 | 285.69 | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| Cellobiose | 5.00 | g | 5 | 14.607 | |
| Wolin's vitamin solution | 2.00 | ml | - | - | |
| Na2S x 9 H2O | 0.40 | g | 0.4 | 1.665 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, bicarbonate, yeast extract, cellobiose, vitamins and sulfide), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate to the medium, dissolve and adjust to pH 9.4. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum bottles and autoclave. To the autoclaved medium add yeast extract, cellobiose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Stock solutions of vitamins and cellobiose should be sterilized by filtration. | |||||
Solution occurs in 166
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 03.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.4 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 24 | 285.69 |
| Cellobiose | 5 | 14.607 |
| Na2CO3 | 2.76 | 26.041 |
| NH4Cl | 1 | 18.695 |
| Yeast extract | 0.5 | - |
| Show more | ||
