DSMZ 1263 . SPIROCHAETA DISSIPATITROPHA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30 513.347
KCl 0.20 g 0.2 2.683
K2HPO4 0.20 g 0.2 1.148
MgCl2 x 6 H2O 0.10 g 0.1 0.492
NH4Cl 1.00 g 1 18.695
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Na2CO3 2.76 g 2.76 26.041
NaHCO3 24.00 g 24 285.69
Yeast extract 0.50 g 0.5 -
Cellobiose 5.00 g 5 14.607
Wolin's vitamin solution 2.00 ml - -
Na2S x 9 H2O 0.40 g 0.4 1.665
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, bicarbonate, yeast extract, cellobiose, vitamins and sulfide), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate to the medium, dissolve and adjust to pH 9.4. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum bottles and autoclave. To the autoclaved medium add yeast extract, cellobiose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Stock solutions of vitamins and cellobiose should be sterilized by filtration.
Solution occurs in 170 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 03.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.4
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 24 285.69
Cellobiose 5 14.607
Na2CO3 2.76 26.041
NH4Cl 1 18.695
Yeast extract 0.5 -
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
pH 10 - 12 1 strain
phyla 1 2 strains
oxygen anaerobe 1 strain
temperature mesophilic 2 strains