This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 30.0 | g | 30 | 513.347 | |
NH4Cl | 0.3 | g | 0.3 | 5.609 | |
CaCl2 x 2 H2O | 0.3 | g | 0.3 | 2.041 | |
MgCl2 x 2 H2O | 3.0 | g | 3 | 22.859 | |
Starch
(soluble) |
5.0 | g | 5 | - | |
Yeast extract | 1.0 | g | 1 | - | |
Peptone
(Difco) |
5.0 | g | 5 | - | |
Phosphate buffer
(10%, pH 7.4) |
10.0 | ml | 1 | - | |
Trace elements (Pfennig) | 1.0 | ml | - | - | |
Resazurin | 0.5 | ml | - | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Boil medium and cool under N2 stream on ice. Check pH (7.4). Autoclave aliquots and add the following sterile solutions (per 10 ml): | |||||
Na2S
(3%) |
0.1 | ml | 0.003 | 0.038 | |
Vitamine B12
(1 mg/10 ml) |
50.0 | µl | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
EDTA | 500 | mg | 0.5 | 1.711 |
FeSO4 x 7 H2O | 200 | mg | 0.2 | 0.719 |
ZnSO4 x 7 H2O | 10 | mg | 0.01 | 0.035 |
H3BO3 | 30 | mg | 0.03 | 0.485 |
CoCl2 x 6 H2O | 20 | mg | 0.02 | 0.154 |
NiCl2 x 6 H2O | 2 | mg | 0.002 | 0.015 |
Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 |
MnCl2 x 4 H2O | 3 | mg | 0.003 | 0.015 |
CuCl2 x 2 H2O | 1 | mg | 1.0e-3 | 0.006 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Medium type: | Complex medium |
Final pH: | 7.4 |
Equipment needed: | Autoclave |
Description:
Not in use anymore! |
|
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 30 | 513.347 |
Peptone | 5 | - |
Starch | 5 | - |
MgCl2 x 2 H2O | 3 | 22.859 |
Yeast extract | 1 | - |
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