1270 .
B12 - MEDIUM GUTTMAN
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Equipment needed:
Final pH:
6.8 - 7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 6.0 | g | ||
|
|
K2HPO4 | 14.0 | g | ||
|
|
Trisodium citrate x 2 H2O | 1.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
DL-Asparagine x H2O | 0.8 | g | ||
|
|
L-Arginine x HCl | 0.2 | g | ||
|
|
L-Glutamic acid | 0.2 | g | ||
|
|
Glycine | 0.2 | g | ||
|
|
L-Histidine | 0.2 | g | ||
|
|
L-Tryptophan | 0.2 | g | ||
|
|
L-Proline | 0.2 | g | ||
|
|
(NH4)2SO4
(don't include in dry mix) |
2.0 | g | ||
|
|
Glucose
(added separately) |
10.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
|
|
Vitamin B12 | 40.0 | µg | ||
| 1 Adjust pH to 6.8 - 7.2. | |||||
| 2 Final concentration of vitamin B12: 40 µg/L. | |||||