DSMZ 1271 . PLEOMORPHOCHAETA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.5 g 0.5 9.348
KH2PO4 0.1 g 0.1 0.735
NaCl 25.0 g 25 427.789
MgCl2 x 6 H2O 4.0 g 4 19.675
CaCl2 x 2 H2O 1.0 g 1 6.802
Yeast extract 1.0 g 1 -
D-Glucose 1.7 g 1.7 9.436
Trace element solution 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
Na2CO3 1.5 g 1.5 14.153
Wolin's vitamin solution 10.0 ml - -
L-Cysteine HCl x H2O 0.3 g 0.3 1.708
Na2S x 9 H2O 0.3 g 0.3 1.249
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except carbonate, vitamins, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 7.3 - 7.5, if necessary.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 4 other media (first defined in Medium 732).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.5 g 0.5 1.487
FeCl2 x 4 H2O 2.0 g 2 10.06
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
AlCl3 10.0 mg 0.01 0.075
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 First dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add remaining compounds.

Metadata

Last modified: 03.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3 - 7.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 5
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 25 427.789
MgCl2 x 6 H2O 4 19.675
D-Glucose 1.7 9.436
Na2CO3 1.5 14.153
CaCl2 x 2 H2O 1 6.802
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Cultivation metadata from 5 strains BacDive

pH 8 - 10 2 strains
pH 6 - 8 2 strains
pH 4 - 6 2 strains
phyla 1 5 strains
nutrition chemoorganotroph 2 strains
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