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DSMZ

DSMZ 1272 . THIOALKALIVIBRIO JANNASCHI

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1272 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 0.5 g 0.5 4.718
NaHCO3 15.0 g 15 178.557
NaCl 15.0 g 15 256.674
K2HPO4 0.5 g 0.5 2.871
Distilled water 1000.0 ml - -
1 Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10.
2 After sterilization add:
Trace element solution 2.0 ml/l - -
MgCl2 x 6 H2O
(200.0 g/l) 		1.0 ml/l 0.2 0.984
3 (a white colloid will form which will rapidly dissolve after mixing)

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 925).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5 mg 0.005 0.017
FeSO4 x 7 H2O 2 mg 0.002 0.007
ZnSO4 x 7 H2O 100 mg 0.1 0.348
MnCl2 x 4 H2O 30 mg 0.03 0.152
CoCl2 x 6 H2O 200 mg 0.2 1.54
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 30 mg 0.03 0.124
CuCl2 x 2 H2O 10 mg 0.01 0.059
H3BO3 300 mg 0.3 4.851
Distilled water 1000 ml - -
1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 10.0
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
NaHCO3 15 178.557
Na2CO3 0.5 4.718
K2HPO4 0.5 2.871
MgCl2 x 6 H2O 0.2 0.984
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain