This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Brucella Broth
(Becton Dickinson) |
2.80 | g | 35 | - | |
Demineralized water | 80.00 | ml | - | - | |
1 Add aseptically to each tube: | |||||
Fetal bovine serum
(heat inactivated) |
2.00 | ml | - | - | |
Vitox
(Oxoid) |
0.06 | ml | - | - | |
Skirrow supplement
(Oxoid) |
0.02 | ml | - | - | |
Amphotericin
(10 mg/ml) |
0.02 | ml | - | - | |
2 Adjust with HCl to pH 5.0, make anoxic under a stream of N2/CO2, distribute in Hungate tubes to 8 ml and autoclave. | |||||
3 Add 1 ml of sterile air to each tube. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 5.0 |
Equipment needed: | Autoclave, Hungate tubes |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Brucella Broth | 35 | - |
Skirrow supplement | - | - |
Vitox | - | - |
Fetal bovine serum | - | - |
Demineralized water | - | - |
Show more |
phyla | 1 | 10 strains |
oxygen | microaerophile | 3 strains |
oxygen | anaerobe | 1 strain |
temperature | mesophilic | 10 strains |