This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Medium 1281 | 75.00 | ml | - | - | |
Salt Solution II | 75.00 | ml | - | - | |
Trypticase
(BBL) |
10.00 | g | 10 | - | |
Yeast extract | 5.00 | g | 5 | - | |
Glucose | 0.25 | g | 0.25 | 1.388 | |
Cellobiose | 0.25 | g | 0.25 | 0.73 | |
Maltose | 0.25 | g | 0.25 | 0.73 | |
Starch
(soluble) |
0.25 | g | 0.25 | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.3 | 1.708 | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
Vitamin solution | 1.00 | ml | - | - | |
Na2CO3
(8% w/v) |
2.50 | ml | 0.2 | 1.887 | |
Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
1 Boil 10 minutes under N2 stream, cool to room temperature. Adjust pH to 7.3 and autoclave for 20 minutes at 121°C. After autoclaving add sterile, anoxic solutions marked with #. For preparation of solid medium add 15.0 g agar per liter. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
K2HPO4 | 6 | g | 6 | 34.448 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KH2PO4 | 6.0 | g | 6 | 44.09 |
(NH4)2SO4 | 12.0 | g | 12 | 90.813 |
NaCl | 12.0 | g | 12 | 205.339 |
MgSO4 x 7 H2O | 1.2 | g | 1.2 | 4.869 |
CaCl2 x 2 H2O | 1.2 | g | 1.2 | 8.162 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 100 | mg | 0.1 | 0.074 |
p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
D-(+)-biotin | 20 | mg | 0.02 | 0.082 |
Nicotinic acid | 200 | mg | 0.2 | 1.625 |
Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 |
Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.3 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 15 | 44.599 |
Trypticase | 10 | - |
Yeast extract | 5 | - |
NaCl | 0.9 | 15.4 |
(NH4)2SO4 | 0.9 | 6.811 |
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