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DSMZ 1281 . ANAEROCELLA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 1281 75.00 ml - -
Salt Solution II 75.00 ml - -
Trypticase
(BBL)
10.00 g 10 -
Yeast extract 5.00 g 5 -
Glucose 0.25 g 0.25 1.388
Cellobiose 0.25 g 0.25 0.73
Maltose 0.25 g 0.25 0.73
Starch
(soluble)
0.25 g 0.25 -
L-Cysteine HCl x H2O 0.30 g 0.3 1.708
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
Vitamin solution 1.00 ml - -
Na2CO3
(8% w/v)
2.50 ml 0.2 1.887
Agar , for solid medium 15.00 g 15 44.599
1 Boil 10 minutesunder N2 stream, cool to room temperature. Adjust pH to 7.3 and autoclave for 20 minutesat 121°C. After autoclaving add sterile, anoxic solutions marked with #. For preparation of solid medium add 15.0 g agar per liter.
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 6 g 6 34.448
Distilled water 1000 ml - -
Solution occurs in 1 other media (first defined in Medium 1281).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 6.0 g 6 44.09
(NH4)2SO4 12.0 g 12 90.813
NaCl 12.0 g 12 205.339
MgSO4 x 7 H2O 1.2 g 1.2 4.869
CaCl2 x 2 H2O 1.2 g 1.2 8.162
Distilled water 1000.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Trypticase 10 -
Yeast extract 5 -
NaCl 0.9 15.4
(NH4)2SO4 0.9 6.811
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain
temperature psychrophilic 1 strain

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