1281 .
ANAEROCELLA MEDIUM
What is this page?
Equipment needed:
Autoclave
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium 1281 | 75.00 | ml | ||
|
|
Salt Solution II | 75.00 | ml | ||
|
|
Trypticase
(BBL) |
10.00 | g | ||
|
|
Yeast extract | 5.00 | g | ||
|
|
Glucose | 0.25 | g | ||
|
|
Cellobiose | 0.25 | g | ||
|
|
Maltose | 0.25 | g | ||
|
|
Starch
(soluble) |
0.25 | g | ||
|
|
L-Cysteine HCl x H2O | 0.30 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Vitamin solution | 1.00 | ml | ||
|
|
Na2CO3
(8% w/v) |
2.50 | ml | ||
|
|
Agar , for solid medium | 15.00 | g | ||
| 1 Boil 10 minutes under N2 stream, cool to room temperature. Adjust pH to 7.3 and autoclave for 20 minutes at 121°C. After autoclaving add sterile, anoxic solutions marked with #. For preparation of solid medium add 15.0 g agar per liter. | |||||
Salt Solution II #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 6.0 | g |
|
|
(NH4)2SO4 | 12.0 | g |
|
|
NaCl | 12.0 | g |
|
|
MgSO4 x 7 H2O | 1.2 | g |
|
|
CaCl2 x 2 H2O | 1.2 | g |
|
|
Distilled water | 1000.0 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 100 | mg |
|
|
p-Aminobenzoic acid | 80 | mg |
|
|
D-(+)-biotin | 20 | mg |
|
|
Nicotinic acid | 200 | mg |
|
|
Calcium pantothenate | 100 | mg |
|
|
Pyridoxine hydrochloride | 300 | mg |
|
|
Thiamine-HCl x 2 H2O | 200 | mg |
|
|
Distilled water | 1000 | ml |