1288
1288 .
RHIZOMICROBIUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1288 #
ml
Equipment needed: Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase
(BBL) |
10.00 | g | 10 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| K2HPO4 | 0.45 | g | 0.45 | 2.584 | |
| KH2PO4 | 0.45 | g | 0.45 | 3.307 | |
| (NH4)2SO4 | 0.90 | g | 0.9 | 6.811 | |
| NaCl | 0.90 | g | 0.9 | 15.4 | |
| MgSO4 x 7 H2O | 0.09 | g | 0.09 | 0.365 | |
| CaCl2 x 2 H2O | 0.09 | g | 0.09 | 0.612 | |
| Na2CO3 | 0.20 | g | 0.2 | 1.887 | |
| L-Cysteine HCl | 0.30 | g | 0.3 | 1.903 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Glucose | 0.25 | g | 0.25 | 1.388 | |
| Cellobiose | 0.25 | g | 0.25 | 0.73 | |
| Maltose | 0.25 | g | 0.25 | 0.73 | |
|
Starch
(soluble) |
0.25 | g | 0.25 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium without glucose, cellobiose, maltose, and soluble starch. Boil the medium and cool under an atmosphere of N2:CO2 (80:20) and adjust the pH to 6.8. Dispense into Hungate tubes or serum bottles under the same gas atmosphere and seal with rubber stoppers. Add the glucose, cellobiose, maltose, and soluble starch from N2/CO2 gassed and sterile-filtered stock solutions (pH 6.8). | |||||
Metadata
| Last modified: | 05.06.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase | 10 | - |
| Yeast extract | 5 | - |
| NaCl | 0.9 | 15.4 |
| (NH4)2SO4 | 0.9 | 6.811 |
| KH2PO4 | 0.45 | 3.307 |
| Show more | ||
