Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 950 | ml | - | - | |
Solution B | 25 | ml | - | - | |
Solution C | 1 | ml | - | - | |
Solution D | 25 | ml | - | - | |
1 Dissolve ingredients of solution A, adjust pH to 8.0 with 1 N NaOH and boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Dispense solution A under same gas atmosphere in anoxic culture vessels (e.g., Hungate-type tubes) and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of solutions B, C and D prepared under 100% N2 gas and sterilized by filtration. Adjust pH of the completed medium to 8.0 with a sterile anoxic solution of 0.1 N HCl or 0.1 N NaOH, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 75.0 | g | 78.947 | 1350.91 |
MgSO4 x 7 H2O | 8.8 | g | 9.263 | 37.582 |
KCl | 1.0 | g | 1.053 | 14.12 |
Yeast extract
(OXOID) |
10.0 | g | 10.526 | - |
Trypticase peptone
(BD BBL) |
10.0 | g | 10.526 | - |
Modified Wolin's mineral solution | 10.0 | ml | - | - |
Distilled water | 940.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
D-Glucose | 2.5 | g | 100 | 555.062 |
Distilled water | 25.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na-thioglycolate | 0.5 | g | 20 | 175.286 |
Na-ascorbate | 0.5 | g | 20 | 100.956 |
Distilled water | 25.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 07.11.22 |
Source: | DSMZ |
Synonyms: | ALKALIPHILUS HALOPHILUS MEDIUM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 8.0 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Trypticase peptone | 9.99 | - |
Yeast extract | 9.99 | - |
D-Glucose | 2.498 | 13.863 |
KCl | 0.999 | 13.4 |
Na-thioglycolate | 0.5 | 4.378 |
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