1291
1291 .
CHTHONOMONAS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1291 #
ml
Equipment needed: Hungate tubes
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Gellan Gum
(Phytagel) |
0.50 | g | 0.5 | - | |
| (NH4)2SO4 | 4.00 | g | 4 | 30.271 | |
| K2HPO4 | 0.45 | g | 0.45 | 2.584 | |
| CaCl2 x 2 H2O | 0.15 | ml | - | - | |
| Trace element solution SL-11 | 1.00 | ml | - | - | |
| MgCl2 x 6 H2O | 1.00 | g | 1 | 4.919 | |
| Yeast extract | 0.01 | g | 0.01 | - | |
| Seven vitamins solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add gellan gum and boil to dissolve. Then add the remaining ingredients (except magnesium chloride, yeast extract and vitamins), adjust the pH value to 5.0 to 5.3 and fill the medium under an air atmosphere into tubes of the Hungate type or similar vials up to 30% their volume. After autoclaving add magnesium chloride, yeast extract and vitamins from steril stock solutions. Vitamins should be sterilized by filtration. | |||||
| 2 Note: Incubation with shaking may improve cell yield. | |||||
Solution occurs in 22
other media (first defined in Medium 722).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA x 2 H2O | 5.2 | g | 5.2 | 15.467 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. | |||||
Solution occurs in 78
other media (first defined in Medium 503).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Vitamin B12 | 100 | mg | 0.1 | 0.074 |
| p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
| D-(+)-biotin | 20 | mg | 0.02 | 0.082 |
| Nicotinic acid | 200 | mg | 0.2 | 1.625 |
| Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
| Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 |
| Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 03.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.0 - 5.3 |
| Equipment needed: | Autoclave, Filter, Hungate tubes |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| (NH4)2SO4 | 4 | 30.271 |
| MgCl2 x 6 H2O | 1 | 4.919 |
| Gellan Gum | 0.5 | - |
| K2HPO4 | 0.45 | 2.584 |
| Yeast extract | 0.01 | - |
| Show more | ||
