DSMZ 12 . SOIL EXTRACT MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Bacillus sp. DSM 352

This strain uses the standard medium without modifications.

Equipment needed: Centrifuge
Compound Amount Unit Conc. [g/L] Conc. [mM]
air-dried garden soil 400 g 400 -
Tap water 1000 ml - -
Agar 15 g 15 44.599
1 Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121°C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8 - 7.0
Equipment needed: Centrifuge
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Associated strains

All strains for this medium: 1
Bacillus sp. DSM 352

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
air-dried garden soil 400 -
Agar 15 44.599
Tap water - -

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain