12
12 .
SOIL EXTRACT MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Bacillus sp. DSM 352
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 12 #
ml
Equipment needed: Centrifuge
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| air-dried garden soil | 400 | g | 400 | - | |
| Tap water | 1000 | ml | - | - | |
| Agar | 15 | g | 15 | 44.599 | |
| 1 Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121°C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.0 |
| Equipment needed: | Centrifuge |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Bacillus sp. DSM 352
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| air-dried garden soil | 400 | - |
| Agar | 15 | 44.599 |
| Tap water | - | - |
