Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
air-dried garden soil | 400 | g | ||
|
Tap water | 1000 | ml | ||
|
Agar | 15 | g | ||
1 Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121°C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize. |