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DSMZ

DSMZ 1300 . ACM-MEDIUM FOR VERMINEPHROBACTER

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1300 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 100 ml - -
1 Autoclave solutions A and B separately and combine after cooling. Adjust pH to 7.2 -7.5.
2 Add Phytagel instead of agar to solidify medium if necessary. Verminephrobacter strains did not grow on plates in our hands.

Solution A #

900
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.5 g 0.556 -
Casamino acids 0.2 g 0.222 -
Na-pyruvate 0.5 g 0.556 5.048
L-Glutamine 0.5 g 0.556 3.802
MgSO4 x 7 H2O 0.3 g 0.333 1.352
MOPS 2.0 g 2.222 10.619
Distilled water 900.0 ml - -

Solution B #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 3 22.045
Distilled water 100.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.2 - 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MOPS 2 9.557
Na-pyruvate 0.5 4.544
L-Glutamine 0.5 3.421
Yeast extract 0.5 -
KH2PO4 0.3 2.205
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