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DSMZ

DSMZ 1303 . MARINE MEDIUM WITH PYRUVATE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1303 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 250 ml - -
Solution B: Artificial sea water 750 ml - -
1 Autoclave solution A and B separately and combine 250 ml Solution A and 750 ml Solution B after cooling. Adjust to pH 7.5.

Solution A #

250
Compound Amount Unit Conc. [g/L] Conc. [mM]
Marine Broth
(i.e. Difco) 		3.7 g 14.8 -
Na-pyruvate 5.0 g 20 181.745
Agar 16.0 g 64 190.289
Distilled water 250.0 ml - -

Solution B: Artificial sea water #

750
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 21.10 g 28.133 481.405
KCl 0.58 g 0.773 10.373
CaCl2 x 2 H2O 1.20 g 1.6 10.883
MgCl2 x 6 H2O 3.60 g 4.8 23.61
NaHCO3 0.08 g 0.107 1.27
MgSO4 x 7 H2O 2.62 g 3.493 14.173
Distilled water 750.00 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 21.1 361.054
Agar 16 47.572
Na-pyruvate 5 45.436
Marine Broth 3.7 -
MgCl2 x 6 H2O 3.6 17.708
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen aerobe 1 strain
temperature mesophilic 1 strain
temperature psychrophilic 1 strain