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1324. MODIFIED METHYLOBACTERIUM MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.495 9.255
2 0.70 g 0.693 3.979
3 0.54 g 0.535 3.929
4 1.00 g 0.99 4.334
5 0.20 g 0.198 1.347
6 4.00 mg 0.004 0.014
7 Trace element solution 10.00 ml - -
8 1000.00 ml 988.324 54860.2
9 0.50 % 5 156.046
10 , might need strain adjustment 15.00 g/l - -
Instructions:
  1. Adjust pH to 6.8. Add sterile methanol to a concentration of 0.5% to the sterile medium. The medium may be solidified with 15 g/l agar
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 300 mg 0.3 4.852
2 200 mg 0.2 0.841
3 100 mg 0.1 0.348
4 30 mg 0.03 0.152
5 10 mg 0.01 0.059
6 20 mg 0.02 0.084
7 60 mg 0.06 0.248
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Methanol 5 156.046
MgSO4 x 6 H2O 0.99 4.334
K2HPO4 0.693 3.979
KH2PO4 0.535 3.929
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain

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