DSMZ 1324 . MODIFIED METHYLOBACTERIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.50 g 0.5 9.348
K2HPO4 0.70 g 0.7 4.019
KH2PO4 0.54 g 0.54 3.968
MgSO4 x 6 H2O 1.00 g 1 8.308
CaCl2 x 2 H2O 0.20 g 0.2 1.36
FeSO4 x 7 H2O 4.00 mg 0.004 0.014
Trace element solution 10.00 ml - -
Distilled water 1000.00 ml - -
Methanol 0.50 % 5 156.045
Agar , for solid medium 15.00 g 15 44.599
1 Adjust pH to 6.8. Add sterile methanol to a concentration of 0.5% to the sterile medium. The medium may be solidified with 15 g/l agar
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 300 mg 0.3 4.851
CoCl2 x 6 H2O 200 mg 0.2 1.54
ZnSO4 x 7 H2O 100 mg 0.1 0.348
MnCl2 x 4 H2O 30 mg 0.03 0.152
CuCl2 x 2 H2O 10 mg 0.01 0.059
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 60 mg 0.06 0.248
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Methanol 5 156.045
MgSO4 x 6 H2O 1 8.308
K2HPO4 0.7 4.019
KH2PO4 0.54 3.968
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain