1325 .
PARACOCCUS BOGORIENSIS MEDIUM
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Equipment needed:
Autoclave
Final pH:
9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 5.0 | g | ||
|
|
Peptone | 5.0 | g | ||
|
|
Glucose | 2.0 | g | ||
|
|
KH2PO4 | 1.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
Na2CO3
(7.5% w/v) |
40.0 | ml | ||
|
|
Trace element solution SL10 | 1.0 | ml | ||
|
|
Distilled water | 960.0 | ml | ||
|
|
Agar , for solid medium | 15.0 | g | ||
| 1 Final pH to 9.5. | |||||
| 2 Autoclave the Na2CO3 solution separately and combine with the rest of the medium after cooling both to about 50°C. Agar may be added at 15g/l to solidify the medium | |||||
Trace element solution SL10 #
1005
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(concentrated) |
5.000 | ml |
|
|
FeCl2 x 4 H2O | 1.500 | g |
|
|
ZnCl2 | 0.070 | g |
|
|
MnCl2 x 4 H2O | 0.200 | g |
|
|
H3BO3 | 6.000 | mg |
|
|
CoCl2 x 6 H2O | 0.190 | g |
|
|
CuCl2 x 2 H2O | 2.000 | mg |
|
|
NiCl2 x 6 H2O | 0.024 | g |
|
|
Na2MoO4 x 2 H2O | 0.036 | g |
|
|
Distilled water | 1000.000 | ml |