133
133 .
CARBON MONOXIDE OXIDIZER MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 133 #
ml
Equipment needed: Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 x 12 H2O | 4.50 | g | 4.5 | 31.699 | |
| KH2PO4 | 0.75 | g | 0.75 | 5.511 | |
| NH4Cl | 1.50 | g | 1.5 | 28.043 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 0.03 | g | 0.03 | 0.204 | |
| Ferric ammonium citrate | 18.00 | mg | 0.018 | 0.069 | |
| Trace element solution SL-6 | 1.00 | ml | - | - | |
|
Na-acetate
optional |
3.00 | g | - | - | |
|
Agar
, for solid medium
optional |
12.00 | g | 12 | 35.679 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients, adjust pH to 7.0 and autoclave. | |||||
| 2 For chemoautotrophic growth incubate under a gas atmosphere of a) 20 - 80% carbon monoxide + 10% O2 + 70 - 10% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.50 g NaHCO3 per liter of medium. | |||||
| 3 For chemoorganotrophic growth add 3.00 g sodium acetate and incubate under air atmosphere. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
| H3BO3 | 0.30 | g | 0.3 | 4.851 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Gassing station |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 12 | 35.679 |
| Na2HPO4 x 12 H2O | 4.5 | 31.699 |
| NH4Cl | 1.5 | 28.043 |
| KH2PO4 | 0.75 | 5.511 |
| MgSO4 x 7 H2O | 0.2 | 0.811 |
| Show more | ||
