This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Meat extract | 20.00 | g | 20 | - | |
NH4Cl | 1.00 | g | 1 | 18.695 | |
MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
Seven vitamins solution | 3.00 | ml | - | - | |
Trace element solution SL-8 | 1.00 | ml | - | - | |
Distilled water | 996.00 | ml | - | - | |
Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
1 Adjust pH to 8.0 before autoclaving. The medium may be solidified by adding 15 g/l agar. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 5.2 | g | 5.2 | 15.467 | |
FeSO4 x 7 H2O | 2.0 | g | 2 | 7.194 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
MnCl2 x 2 H2O | 100.0 | mg | 0.1 | - | |
ZnSO4 x 7 H2O | 150.0 | mg | 0.15 | 0.522 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
H3BO3 | 62.0 | mg | 0.062 | 1.003 | |
CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.1 | |
Distilled water | 1000.0 | ml | - | - | |
1 Adjust pH to 6.8. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin B12 | 100 | mg | 0.1 | 0.074 | |
p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 | |
D-(+)-biotin | 20 | mg | 0.02 | 0.082 | |
Nicotinic acid | 200 | mg | 0.2 | 1.625 | |
Calcium pantothenate | 100 | mg | 0.1 | 0.21 | |
Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 | |
Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 | |
Distilled water | 1000 | ml | - | - | |
1 Filter sterilise and add to the medium after it has been autoclaved. |
Last modified: | 24.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 8.0 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Meat extract | 20 | - |
Agar | 15 | 44.599 |
NH4Cl | 1 | 18.695 |
KH2PO4 | 0.5 | 3.674 |
MgSO4 x 7 H2O | 0.2 | 0.811 |
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