Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 24.0 | g | 24 | 410.678 | |
MgCl2 x 6 H2O | 3.0 | g | 3 | 14.756 | |
MgSO4 x 7 H2O | 2.0 | g | 2 | 8.114 | |
KCl | 0.5 | g | 0.5 | 6.707 | |
CaCl2 | 1.0 | g | 1 | 9.011 | |
Distilled water | 1000.0 | ml | - | - | |
Agar , for solid medium | 20.0 | g | 20 | 59.465 | |
1 Adjust to pH 8.0 and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar. | |||||
2 After autoclaving cool down and add the following to 1 litre medium. Methanol and Vitamine B12 are filter sterilized. Solution T and Wolfe's Mineral Solution are separately autoclaved. Final pH 8.0. | |||||
Methanol | 3.0 | ml | 2.376 | 74.153 | |
Solution T | 20.0 | ml | - | - | |
Wolfe's Mineral Solution | 10.0 | ml | - | - | |
Vitamin B12 solution | 1.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 7 | g | 7 | 51.438 | |
NH4Cl | 100 | g | 100 | 1869.51 | |
Bis-Tris | 100 | g | 100 | 477.92 | |
Ferric ammonium citrate | 3 | g | 3 | - | |
Distilled water | 1000 | ml | - | - | |
1 Adjust to pH 8.0 and sterilize by autoclaving. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 | 3.00 | g | 3 | 24.923 | |
MnSO4 | 0.50 | g | 0.5 | 3.311 | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 | 0.10 | g | 0.1 | 0.658 | |
CaCl2 | 0.10 | g | 0.1 | 0.901 | |
CoCl2 | 0.10 | g | 0.1 | 0.77 | |
ZnSO4 | 0.10 | g | 0.1 | 0.62 | |
CuSO4 | 0.01 | g | 0.01 | 0.063 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 | 0.01 | g | 0.01 | 0.049 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. | |||||
2 Adjust to pH 8.0 with KOH and sterilize by autoclaving. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin B12 | 0.01 | g | 0.1 | 0.074 | |
Distilled water | 100.00 | ml | - | - | |
1 Filter sterilized |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 8.0 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 20 | 59.465 |
MgCl2 x 6 H2O | 3 | 14.756 |
Methanol | 2.376 | 74.153 |
NH4Cl | 2 | 37.39 |
Bis-Tris | 2 | 9.558 |
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