133 .
CARBON MONOXIDE OXIDIZER MEDIUM
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Equipment needed:
Autoclave
Gassing station
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 x 12 H2O | 4.50 | g | ||
|
|
KH2PO4 | 0.75 | g | ||
|
|
NH4Cl | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
CaCl2 x 2 H2O | 0.03 | g | ||
|
|
Ferric ammonium citrate | 18.00 | mg | ||
|
|
Trace element solution SL-6 | 1.00 | ml | ||
|
|
Na-acetate
optional |
3.00 | g | ||
|
|
Agar
, for solid medium
optional |
12.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients, adjust pH to 7.0 and autoclave. | |||||
| 2 For chemoautotrophic growth incubate under a gas atmosphere of a) 20 - 80% carbon monoxide + 10% O2 + 70 - 10% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.50 g NaHCO3 per liter of medium. | |||||
| 3 For chemoorganotrophic growth add 3.00 g sodium acetate and incubate under air atmosphere. | |||||
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |