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DSMZ

DSMZ 134 . HALISCOMENOBACTER MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 134 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 27.00 mg 0.027 0.198
K2HPO4 40.00 mg 0.04 0.23
Na2HPO4 x 2 H2O 40.00 mg 0.04 0.282
CaCl2 x 2 H2O 50.00 mg 0.05 0.34
MgSO4 x 7 H2O 75.00 mg 0.075 0.304
FeCl3 x 6 H2O 5.00 mg 0.005 0.018
MnSO4 x H2O 3.00 mg 0.003 0.018
Trace element solution SL-6 1.00 ml - -
Glutamic acid 1.31 g 1.31 8.904
Trypticase soy broth
(without glucose, BBL) 		2.50 mg 0.003 -
Thiamine-HCl x 2 H2O 0.40 mg 4.0e-4 0.001
Vitamin B12 0.01 mg 1.0e-5 7.4e-6
Glucose 2.00 g 2 11.101
Distilled water 1000.00 ml - -
1 Adjust pH to 7.5. The vitamins are sterilized by filtration. The glucose is dissolved in 5 ml of distilled water and sterilized separately by autoclaving.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 2 11.101
Glutamic acid 1.31 8.904
MgSO4 x 7 H2O 0.075 0.304
CaCl2 x 2 H2O 0.05 0.34
Na2HPO4 x 2 H2O 0.04 0.282
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain