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DSMZ

DSMZ 1341 . MIN E - METHYLOVERSATILIS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1341 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
MIN E - Base (10x) 100 ml - -
Trace Metals (500x) 2 ml - -
Distilled water 900 ml - -
Phosphate buffer (10x) 100 ml - -
Methanol 20 ml 15.84 494.351
Vitamin B12
(250 mg/l) 		1 ml - -
Agar , for solid medium 15 g 15 44.599
1 Add 100 ml of sterile phosphate buffer after autoclaving.
2 Autoclave the Mineral Salts Solution MIN E and phosphate buffer separately and combine after cooling. Add 20 ml/l filter sterilized methanol and 1 ml/l filter sterilized vitamin B12 (250 mg/l). The medium may be solidified by adding 15 g/l agar.

MIN E - Base (10x) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 6 H2O 0.50 g 0.5 2.282
MgSO4 x 7 H2O 1.65 g 1.65 6.694
(NH4)2SO4 5.00 g 5 37.839
Distilled water 1000.00 ml - -

Trace Metals (500x) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 9.00 g 9 225.017
EDTA 50.00 g 50 171.092
ZnSO4 x 7 H2O 11.00 g 11 38.253
CaCl2 x 2 H2O 7.34 g 7.34 49.927
MnCl2 x 4 H2O 2.50 g 2.5 12.632
CoCl2 x 6 H2O 0.50 g 0.5 3.851
(NH4)6Mo7O24 0.50 g 0.5 -
FeSO4 x 7 H2O 5.00 g 5 17.985
CuSO4 x 5 H2O 0.20 g 0.2 0.801
Distilled water 1000.00 ml - -
1 Dissolve the EDTA and NaOH in 800ml of distilled water then add the inorganic salts. Make up to 1000.0 ml with distilled water.

Phosphate buffer (10x) #

1000
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 12 g 12 68.896
KH2PO4 6 g 6 44.09
Distilled water 1000 ml - -
1 pH 6.8 - 7.0

Metadata

Last modified: 31.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Methanol 15.84 494.351
Agar 15 44.599
K2HPO4 1.2 6.89
KH2PO4 0.6 4.409
(NH4)2SO4 0.5 3.784
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen obligate aerobe 2 strains
oxygen aerobe 1 strain
temperature mesophilic 4 strains