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DSMZ

DSMZ 1352 . FUCHSIELLA ALKALIACETIGENA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1352 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.196 1.439
MgCl2 x 6 H2O 0.10 g 0.098 0.482
NH4Cl 0.50 g 0.49 9.155
KCl 0.20 g 0.196 2.628
NaCl 60.00 g 58.766 1005.58
Na2CO3 68.30 g 66.895 631.158
NaHCO3 38.30 g 37.512 446.537
Trace element solution SL-4 1.00 ml - -
Resazurin 0.50 mg 4.9e-4 0.002
Wolin's vitamin solution 10.00 ml - -
Yeast extract 0.05 g 0.049 -
Ethanol
(50% v/v) 		10.00 ml - -
Na2S x 9 H2O 1.00 g 0.979 4.078
NaNO3 0.85 g 0.833 9.795
Distilled water 1000.00 ml - -
1 pH 8.8- 9.5; gas atmosphere: 100% N2.
2 Dissolve ingredients except the sodium carbonate, sodium bicarbonate, ethanol, and sodium sulphide. Boil medium for a few minutes and cool to room temperature while flushing with N2. Add and dissolve sodium carbonate, sodium bicarbonate and sodium sulphide while gassing the head space only. Dispense and autoclave under N2. Before use add 10 ml/l (v/v) of 50% (v/v) ethanol solution (flushed and autoclaved under N2).

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.8 - 9.5
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 66.895 631.158
NaCl 58.766 1005.58
NaHCO3 37.512 446.537
Na2S x 9 H2O 0.979 4.078
NaNO3 0.833 9.795
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Cultivation metadata from 3 strains BacDive

pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 3 strains
oxygen anaerobe 1 strain
temperature mesophilic 2 strains
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