1352 .
FUCHSIELLA ALKALIACETIGENA MEDIUM
What is this page?
Equipment needed:
Final gas composition:
100 % N2
read more
Final pH:
8.8 - 9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.20 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
|
KCl | 0.20 | g | ||
|
|
NaCl | 60.00 | g | ||
|
|
Na2CO3 | 68.30 | g | ||
|
|
NaHCO3 | 38.30 | g | ||
|
|
Trace element solution SL-4 | 1.00 | ml | ||
|
|
Resazurin | 0.50 | mg | ||
|
|
Wolin's vitamin solution | 10.00 | ml | ||
|
|
Yeast extract | 0.05 | g | ||
|
|
Ethanol
(50% v/v) |
10.00 | ml | ||
|
|
Na2S x 9 H2O | 1.00 | g | ||
|
|
NaNO3 | 0.85 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 8.8- 9.5; gas atmosphere: 100% N2. | |||||
| 2 Dissolve ingredients except the sodium carbonate, sodium bicarbonate, ethanol, and sodium sulphide. Boil medium for a few minutes and cool to room temperature while flushing with N2. Add and dissolve sodium carbonate, sodium bicarbonate and sodium sulphide while gassing the head space only. Dispense and autoclave under N2. Before use add 10 ml/l (v/v) of 50% (v/v) ethanol solution (flushed and autoclaved under N2). | |||||
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
EDTA | 0.5 | g |
|
|
FeSO4 x 7 H2O | 0.2 | g |
|
|
Trace element solution SL-6 | 100.0 | ml |
|
|
Distilled water | 900.0 | ml |
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium D-(+)-pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |