This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract | 1.00 | g | 1 | - | |
Tryptone | 5.00 | g | 5 | 70.343 | |
Casamino acids | 5.00 | g | 5 | - | |
Beef extract
(Lab-Lemco-Powder) |
5.00 | g | 5 | - | |
K2HPO4 | 1.00 | g | 1 | 5.741 | |
NH4NO3 | 1.00 | g | 1 | - | |
Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
Cysteine HCl | 0.25 | g | 0.25 | 2.063 | |
NiCl2 x 6 H2O | 7.50 | mg | 0.008 | 0.058 | |
Trace element solution | 5.00 | ml | - | - | |
Resazurin
(0.1% v/v) |
0.50 | ml | - | - | |
Artifical seawater solution | 250.00 | ml | - | - | |
Distilled water | 750.00 | ml | - | - | |
1 Prepare the liquid medium without Na2S and cysteine-HCl. Boil the medium in a water bath and cool under N2:CO2 (80:20 v/v) to room temperature. Adjust to pH 7.0 with 10 M NaOH solution. Dispense the medium into Hungate tubes or serum bottles. Sterilize by autoclaving. Before inoculatiing add Na2S and cysteine-HCl from sterile stock solutions. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
FePO4 | 1.00 | g | 2 | 13.261 |
Na2WO4 x 2 H2O | 0.01 | g | 0.02 | 0.061 |
Na2SO3 x 5 H2O | 0.01 | g | 0.02 | 0.159 |
Distilled water | 500.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 20.00 | g | 20 | 342.231 |
MgCl2 x 6 H2O | 3.00 | g | 3 | 14.756 |
MgSO4 x 7 H2O | 6.00 | g | 6 | 24.343 |
NaHCO3 | 0.20 | g | 0.2 | 2.381 |
CaCl2 x 2 H2O | 0.30 | g | 0.3 | 2.041 |
KCl | 0.50 | g | 0.5 | 6.707 |
KH2PO4 | 0.42 | g | 0.42 | 3.086 |
NaBr | 0.05 | g | 0.05 | 0.486 |
SrCl2 | 0.02 | g | 0.02 | 0.126 |
Fe(NH4)citrate | 0.01 | g | 0.01 | - |
(NH4)2SO4 | 1.00 | g | 1 | 7.568 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 5 | 85.558 |
Tryptone | 5 | 70.343 |
Casamino acids | 5 | - |
Beef extract | 5 | - |
MgSO4 x 7 H2O | 1.5 | 6.086 |
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