DSMZ 1353 . YTBC - MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.00 g 1 -
Tryptone 5.00 g 5 70.343
Casamino acids 5.00 g 5 -
Beef extract
(Lab-Lemco-Powder)
5.00 g 5 -
K2HPO4 1.00 g 1 5.741
NH4NO3 1.00 g 1 -
Na2S x 9 H2O 0.25 g 0.25 1.041
Cysteine HCl 0.25 g 0.25 2.063
NiCl2 x 6 H2O 7.50 mg 0.008 0.058
Trace element solution 5.00 ml - -
Resazurin
(0.1% v/v)
0.50 ml - -
Artifical seawater solution 250.00 ml - -
Distilled water 750.00 ml - -
1 Prepare the liquid medium without Na2S and cysteine-HCl. Boil the medium in a water bath and cool under N2:CO2 (80:20 v/v) to room temperature. Adjust to pH 7.0 with 10 M NaOH solution. Dispense the medium into Hungate tubes or serum bottles. Sterilize by autoclaving. Before inoculatiing add Na2S and cysteine-HCl from sterile stock solutions.
Compound Amount Unit Conc. [g/L] Conc. [mM]
FePO4 1.00 g 2 13.261
Na2WO4 x 2 H2O 0.01 g 0.02 0.061
Na2SO3 x 5 H2O 0.01 g 0.02 0.159
Distilled water 500.00 ml - -
Equipment needed: Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 20 342.231
MgCl2 x 6 H2O 3.00 g 3 14.756
MgSO4 x 7 H2O 6.00 g 6 24.343
NaHCO3 0.20 g 0.2 2.381
CaCl2 x 2 H2O 0.30 g 0.3 2.041
KCl 0.50 g 0.5 6.707
KH2PO4 0.42 g 0.42 3.086
NaBr 0.05 g 0.05 0.486
SrCl2 0.02 g 0.02 0.126
Fe(NH4)citrate 0.01 g 0.01 -
(NH4)2SO4 1.00 g 1 7.568
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 5 85.558
Tryptone 5 70.343
Casamino acids 5 -
Beef extract 5 -
MgSO4 x 7 H2O 1.5 6.086
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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