DSMZ 1355 . HYPHOMICROBIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 2.50 g 2.5 14.353
NaH2PO4 2.25 g 2.25 18.754
(NH4)2SO4 0.50 g 0.5 3.784
MgSO4 x 7 H2O 0.20 g 0.2 0.811
Visniac Trace Elements (1000x) 1.00 ml - -
Distilled water 1000.00 ml - -
Agar , for solid medium 12.00 g 12 35.679
Methylamine
(0.2% v/v)
2.00 ml - -
1 Adjust to pH 6 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar. After autoclaving add sterile methylamine (0.2% v/v) to the sterile main solution.
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.000 g 10 34.219
ZnSO4 x 7 H2O 2.200 g 4.4 15.301
CaCl2 x 2 H2O 0.733 g 1.466 9.972
MnCl2 x 4 H2O 0.506 g 1.012 5.114
FeSO4 x 7 H2O 0.499 g 0.998 3.59
(NH4)6Mo7O24 x 4 H2O 0.110 g 0.22 -
CuSO4 x 5 H2O 0.157 g 0.314 1.258
CoCl2 x 6 H2O 0.161 g 0.322 2.48
Distilled water 500.000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.0
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 12 35.679
K2HPO4 2.5 14.353
NaH2PO4 2.25 18.754
(NH4)2SO4 0.5 3.784
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain
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