1355 .
HYPHOMICROBIUM MEDIUM
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Equipment needed:
Final pH:
6.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 2.50 | g | ||
|
|
NaH2PO4 | 2.25 | g | ||
|
|
(NH4)2SO4 | 0.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
Visniac Trace Elements (1000x) , pH 6 | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Agar , for solid medium | 12.00 | g | ||
|
|
Methylamine
(0.2% v/v) |
2.00 | ml | ||
| 1 Adjust to pH 6 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar. After autoclaving add sterile methylamine (0.2% v/v) to the sterile main solution. | |||||
Visniac Trace Elements (1000x) #
500
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.000 | g | ||
|
|
ZnSO4 x 7 H2O | 2.200 | g | ||
|
|
CaCl2 x 2 H2O | 0.733 | g | ||
|
|
MnCl2 x 4 H2O | 0.506 | g | ||
|
|
FeSO4 x 7 H2O | 0.499 | g | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 0.110 | g | ||
|
|
CuSO4 x 5 H2O | 0.157 | g | ||
|
|
CoCl2 x 6 H2O | 0.161 | g | ||
|
|
Distilled water | 500.000 | ml | ||
| 1 Adjust pH to 6.0 with NaOH. | |||||