1357
1357 .
MAB MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1357 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 10.000 | g | 10 | 171.116 | |
| NH4Cl | 0.850 | g | 0.85 | 15.891 | |
| K2HPO4 | 0.480 | g | 0.48 | 2.756 | |
| MgSO4 x 7 H2O | 0.120 | g | 0.12 | 0.487 | |
| CaCl2 x 2 H2O | 0.061 | g | 0.061 | 0.415 | |
| FeSO4 x 7 H2O | 0.021 | g | 0.021 | 0.076 | |
| MnSO4 x H2O | 5.000 | mg | 0.005 | 0.03 | |
| CoCl2 x 6 H2O | 1.000 | mg | 1.0e-3 | 0.008 | |
| ZnSO4 x 7 H2O | 1.000 | mg | 1.0e-3 | 0.003 | |
| Trace element solution | 1.000 | ml | - | - | |
| Vitamin solution | 10.000 | ml | - | - | |
| Resazurin | 1.000 | µg | 1.0e-6 | 4.4e-6 | |
| Na2CO3 | 10.600 | g | 10.6 | 100.011 | |
| NaHCO3 | 13.600 | g | 13.6 | 161.891 | |
| Cysteine HCl | 0.700 | g | 0.7 | 5.777 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Prepare the liquid medium without Na2CO3, NaHCO3, Vitamin solution and cysteine HCl. Boil and then cool under N2 to room temperature. Add Na2CO3 and NaHCO3. Dispense into suitable vessels for anaerobic growth (serum bottles or Hungate tubes sealed with rubber stoppers) and sterilize by autoclaving. After autoclaving add cysteine HCl from sterile stock solution and 10 ml of the vitamin solution to 1 litre of the sterile main solution. Final pH 9.8. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CuSO4 x 2 H2O | 0.1 | g | 0.1 | 0.627 |
| Nitrilotriacetic acid | 15.0 | g | 15 | 78.477 |
| AlK(SO4)2 x 12 H2O | 0.1 | g | 0.1 | 0.211 |
| H3BO3 | 0.1 | g | 0.1 | 1.617 |
| Na2MoO4 x 2 H2O | 0.1 | g | 0.1 | 0.413 |
| Distilled water | 1000.0 | ml | - | - |
Equipment needed: Hungate tubes
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Pyridoxine hydrochloride | 0.10 | mg | 1.0e-4 | 4.9e-4 |
| Thiamine HCl | 0.05 | mg | 5.0e-5 | 1.5e-4 |
| Riboflavin | 0.05 | mg | 5.0e-5 | 1.3e-4 |
| Nicotinic acid | 0.05 | mg | 5.0e-5 | 4.1e-4 |
| p-Aminobenzoic acid | 0.05 | mg | 5.0e-5 | 3.6e-4 |
| Lipoic acid | 0.05 | mg | 5.0e-5 | 2.4e-4 |
| Biotin | 0.02 | mg | 2.0e-5 | 8.2e-5 |
| Folic acid | 0.02 | mg | 2.0e-5 | 4.5e-5 |
| Vitamin B12 | 5.00 | µg | 5.0e-6 | 3.7e-6 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 16.01.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.8 |
| Equipment needed: | Anaerobic workbench, Hungate tubes |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 13.6 | 161.891 |
| Na2CO3 | 10.6 | 100.011 |
| NaCl | 10 | 171.116 |
| NH4Cl | 0.85 | 15.891 |
| Cysteine HCl | 0.7 | 5.777 |
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