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DSMZ

DSMZ 1359 . PHAEOVIBRIO SULPHIDIPHILUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1359 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.5 3.674
MgSO4 x 7 H2O 0.50 g 0.5 2.029
NaCl 0.40 g 0.4 6.845
NH4Cl 0.68 g 0.68 12.713
CaCl2 x 2 H2O 0.25 g 0.25 1.701
Na pyruvate 0.50 g 0.5 4.544
Sodium succinate 0.50 g 0.5 3.085
Na acetate 0.50 g 0.5 6.095
Yeast extract 0.50 g 0.5 -
Ferric citrate
(0.1%) 		5.00 ml 0.005 0.02
NaHCO3
(10%) 		15.00 ml 1.5 17.856
Na2S x 9 H2O
(1M) 		1.00 ml 0.24 1
Vitamin B12
(2mg/100ml) 		1.00 ml - -
Trace element solution SL7 1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the basal medium under nitrogen.
2 NaHCO3 solution (autoclaved in sealed, half full vessels under CO2), Na2S x 9H2O solution (autoclaved) and vitamin B12 solution (filter sterilised) is added to the autoclaved medium and the final pH adjusted to 6.6 - 7.4.

Trace element solution SL7 #

1001
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, v/v) 		1 ml - -
ZnCl2 70 mg 0.07 0.513
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 60 mg 0.06 0.969
CoCl2 x 6 H2O 200 mg 0.2 1.539
CuCl2 x 2 H2O 20 mg 0.02 0.117
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 40 mg 0.04 0.165
Distilled water 1000 ml - -

Metadata

Last modified: 31.03.22
Source: DSMZ
Medium type: Complex medium
Final pH: 6.6 - 7.4
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
NH4Cl 0.68 12.713
Na acetate 0.5 6.095
Na pyruvate 0.5 4.544
KH2PO4 0.5 3.674
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Cultivation metadata from 0 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain