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135a. ACETOBACTERIUM MEDIUM (MARINE)

Culture medium recipe

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* Modified for Acetobacterium sp. DSM 2396
beta

Anaerobic

Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 19.418 332.249
2 1.00 g 0.971 18.15
3 0.33 g 0.32 2.354
4 0.45 g 0.437 2.508
5 0.10 g 0.097 0.394
6 Trace element solution 20.00 ml - -
7 2.00 g 1.942 -
8
(0.1% w/v)
0.50 ml 4.9e-4 -
9 5.00 g 4.854 57.786
10 1.25 g 1.214 19.553
11 Vitamin solution 10.00 ml - -
12 0.50 g 0.485 2.764
13 0.50 g 0.485 2.021
14 1000.00 ml 969.133 53795
Instructions:
  1. Dissolve ingredients except bicarbonate, ethylene glycol, vitamins, cysteine and sulfide, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add bicarbonate (solid) and equilibrate the medium with the gas until a pH of around 7.4 is reached. Then distribute under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add ethylene glycol, vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.0 - 7.2, if necessary.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
17 , required for pH adjustment x - -
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1
Acetobacterium sp. DSM 2396

Gas composition

  • 100 % N2

Light

Intensity lux

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 19.437 332.581
NaHCO3 4.854 57.786
Yeast extract 1.942 -
Ethylene glycol 1.214 19.553
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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