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DSMZ

DSMZ 135b . ACETOBACTERIUM DEHALOGENANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 135b #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 0.994 18.584
KH2PO4 0.33 g 0.328 2.41
K2HPO4 0.45 g 0.447 2.568
MgSO4 x 7 H2O 0.10 g 0.099 0.403
Modified Wolin's mineral solution 20.00 ml - -
Yeast extract 2.00 g 1.988 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 1.50 g 1.491 14.068
Na-syringate solution (0.3 M) 15.00 ml - -
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.497 2.83
Distilled water 970.00 ml - -
1 Dissolve ingredients except for carbonate, Na-syringate solution, vitamins, and cysteine, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Thereafter, dispense under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add Na-syringate solution, vitamins, and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the completed medium should be at 7.4.
2 Note: After growth has been started add an additional amount of Na-syringate to reach a final concentration of 2.00 g/l.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Na-syringate solution (0.3 M) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Syringic acid
(SIGMA S6881) 		60 g 60 -
NaOH
(5 N) 		50 ml - -
Distilled water 950 ml - -
1 Add syringic acid to 700 ml distilled water, add about 50 ml of 5 N NaOH and adjust the pH to about 7.5 to dissolve it. Then make up to a volume of 1000 ml with water. Make the solution anoxic by sparging with 100% N2 gas, distribute in anoxic serum vials and autoclave at 121°C.

Metadata

Last modified: 24.04.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.988 -
Na2CO3 1.491 14.068
NH4Cl 0.994 18.584
Syringic acid 0.895 -
L-Cysteine HCl x H2O 0.497 2.83
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Cultivation metadata from 0 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain