1361 .
PLANCTOMYCETALES BACTERIUM MEDIUM
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Equipment needed:
Autoclave
Hungate tubes
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.14 | g | ||
|
|
MgCl2 x 6 H2O | 0.20 | g | ||
|
|
CaCl2 x 2 H2O | 0.15 | g | ||
|
|
NH4Cl | 0.54 | g | ||
|
|
Trace elements Solution | 1.00 | ml | ||
|
|
Glucose | 0.18 | g | ||
|
|
Yeast extract | 0.20 | g | ||
|
|
Vitamin solution | 1.00 | ml | ||
|
|
Resazurin
(0.1 v/v) |
0.50 | ml | ||
|
|
NaHCO3 | 2.50 | g | ||
|
|
Na2S x 9 H2O | 0.30 | g | ||
|
|
Cysteine HCl | 0.30 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride, vitamin solution and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v). Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v) and add the NaHCO3. The final pH should be 7.0. Autoclave the medium and add from sterile stock solutions yeast extract, glucose, cysteine hydrochloride, vitamin solution and Na2S x 9 H2O.The final pH should be 7.0. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine-HCl x 2 H2O | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
D-Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace elements Solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Nitrilotriacetic acid
(NTA) |
12.800 | g |
|
|
FeCl3 x 6 H2O | 1.350 | g |
|
|
MnCl2 x 4 H2O | 0.100 | g |
|
|
CoCl2 x 6 H2O | 0.024 | g |
|
|
CaCl2 x 2 H2O | 0.100 | g |
|
|
ZnCl2 | 0.100 | g |
|
|
CuCl2 x 2 H2O | 0.025 | g |
|
|
H3BO3 | 0.010 | g |
|
|
Na2MoO4 x 2 H2O | 0.024 | g |
|
|
NiCl2 x 6 H2O | 0.120 | g |
|
|
Na2SeO3 x 5 H2O | 4.000 | mg |
|
|
Distilled water | 1000.000 | ml |