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1369. ECTOTHIORHODOSPIRA MAGNA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 3.671
2 30.0 g 29.97 512.812
3 0.5 g 0.5 9.338
4 0.2 g 0.2 0.983
5 1.0 g 0.999 12.178
6 0.5 g 0.5 3.159
7 0.5 g 0.5 2.08
8 5.0 g 4.995 59.46
9 5.0 g 4.995 47.128
10 0.1 g 0.1 -
11 10.0 µg 1.0e-5 6.3e-6
12 Trace elements solution (Pfennig & Lippert,1966) 1.0 ml - -
13 1000.0 ml 997.21 55353.5
Instructions:
  1. pH 9.0-9.5
  2. Prepare the medium without the NaHCO3, Na2S x 9 H2O, Na2CO3 and vitamin B12, under a nitrogen atmosphere.
  3. Autoclave and add the vitamin B12 from a filter-sterilised stock solution and the NaHCO3 (autoclaved in sealed, half full vessels), Na2S x 9 H2O, Na2CO3 from sterile stock solutions. The final pH of the medium should be 9.0-9.5.
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.2
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
NaCl 29.97 512.812
NaHCO3 4.995 59.46
Na2CO3 4.995 47.128
Sodium acetate 0.999 12.178
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

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