1369
1369 .
ECTOTHIORHODOSPIRA MAGNA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1369 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| NaCl | 30.0 | g | 30 | 513.347 | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| Na-acetate | 1.0 | g | 1 | 12.19 | |
| Na2S2O3 | 0.5 | g | 0.5 | 1.167 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| NaHCO3 | 5.0 | g | 5 | 59.519 | |
| Na2CO3 | 5.0 | g | 5 | 47.175 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Vitamin B12 | 10.0 | µg | 1.0e-5 | 7.4e-6 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 pH 9.0-9.5 | |||||
| 2 Prepare the medium without the NaHCO3, Na2S x 9 H2O, Na2CO3 and vitamin B12, under a nitrogen atmosphere. | |||||
| 3 Autoclave and add the vitamin B12 from a filter-sterilised stock solution and the NaHCO3 (autoclaved in sealed, half full vessels), Na2S x 9 H2O, Na2CO3 from sterile stock solutions. The final pH of the medium should be 9.0-9.5. | |||||
Solution occurs in 28
other media (first defined in Medium 1369).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2.20 | g | 2.2 | 7.913 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.03 | g | 0.03 | 0.485 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.03 | g | 0.03 | 0.176 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 3.0-4.0 | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 - 9.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 30 | 513.347 |
| NaHCO3 | 5 | 59.519 |
| Na2CO3 | 5 | 47.175 |
| Na-acetate | 1 | 12.19 |
| NH4Cl | 0.5 | 9.348 |
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