DSMZ 137 . MINERAL LACTATE MEDIUM (low carbon content)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-lactate 0.50 g 0.5 4.462
K2HPO4 x 3 H2O 1.13 g 1.13 6.488
KH2PO4 0.88 g 0.88 6.467
NH4Cl 1.00 g 1 18.695
MgSO4 x 7 H2O 0.50 g 0.5 2.029
NaCl 1.00 g 1 17.112
CaCl2 x 2 H2O 5.00 mg 0.005 0.034
Trace element solution (Vishniac and Santer, 1957) 1.20 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0.
Solution occurs in 4 other media (first defined in Medium 69).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 22.00 g 22 76.506
CaCl2 x 2 H2O 5.54 g 5.54 37.683
MnCl2 x 4 H2O 5.06 g 5.06 25.568
FeSO4 x 7 H2O 5.00 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.10 g 1.1 0.89
CuSO4 x 5 H2O 1.57 g 1.57 6.288
CoCl2 x 6 H2O 1.61 g 1.61 12.4
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
K2HPO4 x 3 H2O 1.13 6.488
NH4Cl 1 18.695
NaCl 1 17.112
KH2PO4 0.88 6.467
Na-lactate 0.5 4.462
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain