Name: IRD MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1373 #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.3	g	0.3	2.205
K₂HPO₄	0.3	g	0.3	1.722
NaCl	1.0	g	1	17.112
NH₄Cl	1.0	g	1	18.695
MgCl₂ x 6 H₂O	0.5	g	0.5	2.459
CaCl₂ x 2 H₂O	0.1	g	0.1	0.68
KCl	0.1	g	0.1	1.341
Yeast extract	1.0	g	1	-
Tryptone	1.0	g	1	14.069
Cysteine HCl	0.5	g	0.5	4.127
Modified Wolin's mineral solution II	1.0	ml	-	-
Resazurin (0.1%)	0.5	ml	5.0e-4	0.002
Distilled water	1000.0	ml	-	-
1 Autoclave and add the following components after autoclaving from sterile stock solutions:
NaHCO₃ (10%)	10.0	ml	1	11.904
Na₂S (2%)	10.0	ml	0.2	2.53
Glucose (1 M)	10.0	ml	1.802	10
2 pH 7.2
3 Prepare the medium under a N₂/CO₂ atmosphere.
4 The final pH of the medium should be 7.2.

Modified Wolin's mineral solution II #

1000

Solution occurs in 24 other media (first defined in Medium 700).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.500	g	1.5	7.848
MgSO₄ x 7 H₂O	3.000	g	3	12.172
MnSO₄ x H₂O	0.500	g	0.5	2.958
NaCl	1.000	g	1	17.112
FeSO₄ x 7 H₂O	0.100	g	0.1	0.36
CoSO₄ x 7 H₂O	0.180	g	0.18	1.161
CaCl₂ x 2 H₂O	0.100	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.180	g	0.18	0.626
CuSO₄ x 5 H₂O	0.010	g	0.01	0.04
AlK(SO₄)₂ x 12 H₂O	0.020	g	0.02	0.042
H₃BO₃	0.010	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.010	g	0.01	0.041
NiCl₂ x 6 H₂O	0.025	g	0.025	0.193
Na₂SeO₃ x 5 H₂O	0.300	mg	3.0e-4	0.001
Distilled water	1000.000	ml	-	-
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

All strains for this medium:

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phyla	1	1 strain
temperature	thermophilic	1 strain