1374 .
IGNAVIBACTERIA MEDIUM
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Selected strain: Melioribacter roseus DSM 23840
This strain uses the standard medium without modifications.
Growth conditions: 7-14 days
anaerobic
47 °C
Equipment needed:
Autoclave
Filter
Final pH:
7.2 - 7.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 1.00 | g | ||
|
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MgCl2 x 6 H2O | 0.40 | g | ||
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KCl | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
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NH4Cl | 0.25 | g | ||
|
|
KH2PO4 | 0.20 | g | ||
|
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Na2SO4 | 4.00 | g | ||
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Trace element solution | 1.00 | ml | ||
|
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Yeast extract | 2.00 | g | ||
|
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NaHCO3 | 0.10 | g | ||
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Distilled water | 990.00 | ml | ||
| 1 Autoclave at 121 °C for 15 min. | |||||
| 2 Add filter-sterilized vitamin solution (1ml/10ml) | |||||
|
|
Vitamin solution | 10.00 | ml | ||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Fe(NH4)2(SO4)2 x 6 H2O | 784.0 | mg |
|
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ZnSO4 x 7 H2O | 143.6 | mg |
|
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MnCl2 x 4 H2O | 99.0 | mg |
|
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Na2SeO4 | 94.6 | mg |
|
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H3BO3 | 6.0 | mg |
|
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Na2WO4 x 2 H2O | 33.0 | mg |
|
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CuCl2 x 2 H2O | 1.8 | mg |
|
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CoCl2 x 6 H2O | 238.0 | mg |
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(NH4)2Ni(SO4)2 x 6 H2O | 395.0 | mg |
|
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Na2MoO4 x 2 H2O | 24.0 | mg |
|
|
HCl
(concentrated) |
10.0 | ml |
|
|
Distilled water | 990.0 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
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Pyridoxine hydrochloride | 10.0 | mg |
|
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Thiamine-HCl x 2 H2O | 5.0 | mg |
|
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Riboflavin | 5.0 | mg |
|
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Nicotinic acid | 5.0 | mg |
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D-Calcium pantothenate | 5.0 | mg |
|
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Vitamin B12 | 0.1 | mg |
|
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p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |